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. 2004 Nov 1;10(21):3161-4.
doi: 10.3748/wjg.v10.i21.3161.

Protective effects of tumor necrosis factor alpha antibody and ulinastatin on liver ischemic reperfusion in rats

Yan-Ling Yang  1 , Ji-Peng Li, Xiao-Ping Xu, Ke-Feng Dou, Shu-Qiang Yue, Kai-Zong Li
Affiliations

Protective effects of tumor necrosis factor alpha antibody and ulinastatin on liver ischemic reperfusion in rats

Yan-Ling Yang et al. World J Gastroenterol. .

Abstract

Aim: To study the protective effects of tumor necrosis factor alpha (TNF alpha) antibody and ulinastatin on liver ischemic reperfusion in rats.

Methods: One hundred and twenty male SD rats were randomly divided into four groups: normal control group, ischemic group, TNF alpha antibody group and TNF alpha antibody + ulinastatin group. The animals were killed at 0, 3, 6, 9, 12 h after ischemia for 60 min and followed by reperfusion. Serum alanine aminotransferase (ALT), malondialdehyde (MDA) and liver histopathology were observed.

Results: After ischemic reperfusion, the serum ALT and MDA were remarkably increased, and the hepatic congestion was obvious. Treatment of TNF alpha antibody and ulinastatin could significantly decrease serum ALT and MDA levels, and relieve hepatic congestion.

Conclusion: Ulinastatin and TNF alpha antibody can suppress the inflammatory reaction induced by hepatic ischemic reperfusion, and have protective effects on rat hepatic ischemic reperfusion injury.

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Figures

Figure 1
Figure 1
Serum ALT level after reperfusion in rats.
Figure 2
Figure 2
Serum MDA level after reperfusion in rats.
Figure 3
Figure 3
Disorganized hepatic lobules and extensive hepatocytic edema with various degrees of vacuolation and lamellar ne-crosis in ischemic reperfusion injury group.
Figure 4
Figure 4
A sprinkle of hepatocytic edema without vacuola-tion and lamellar necrosis in TNFα antibody + ulinastatin treatment group.
See this image and copyright information in PMC

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