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. 2004 Oct;70(10):6315-9.
doi: 10.1128/AEM.70.10.6315-6319.2004.

Metabolism of 2-mercaptobenzothiazole by Rhodococcus rhodochrous

Nicolas Haroune  1 , Bruno Combourieu, Pascale Besse, Martine Sancelme, Achim Kloepfer, Thorsten Reemtsma, Heleen De Wever, Anne-Marie Delort
Affiliations

Metabolism of 2-mercaptobenzothiazole by Rhodococcus rhodochrous

Nicolas Haroune et al. Appl Environ Microbiol. 2004 Oct.

Abstract

2-Mercaptobenzothiazole, which is mainly used in the rubber industry as a vulcanization accelerator, is very toxic and is considered to be recalcitrant. We show here for the first time that it can be biotransformed and partially mineralized by a pure-culture bacterial strain of Rhodococcus rhodochrous. Three metabolites, among four detected, were identified.

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Figures

FIG. 1.
FIG. 1.
In situ 1H NMR spectra of samples taken at 1, 2, 3, 4, and 128 h of incubation of 1.5 mM MBT with resting cells of R. rhodochrous OBT18.
FIG. 2.
FIG. 2.
1H-15N gHMBC spectrum of purified 6-hydroxy-MBT recorded in methanol; the experiment was set up for assumed 3J (delay, 80 ms) long-range coupling. The spots visible on the 2D spectrum correspond to the correlations between N3 and H4 (3JH4N3). The presence of a proton at position 5 of the benzene ring demonstrates that the hydroxyl group is at position 6.
FIG. 3.
FIG. 3.
Proposed metabolic pathway of MBT in R. rhodochrous OBT18.
See this image and copyright information in PMC

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