Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Oct;42(10):4494-7.
doi: 10.1128/JCM.42.10.4494-4497.2004.

Genotyping of 44 isolates of Fusarium solani, the main agent of fungal keratitis in Brazil

Patricio Godoy  1 , Josep Cano, Josepa Gené, Josep Guarro, Ana Luisa Höfling-Lima, Arnaldo Lopes Colombo
Affiliations

Genotyping of 44 isolates of Fusarium solani, the main agent of fungal keratitis in Brazil

Patricio Godoy et al. J Clin Microbiol. 2004 Oct.

Abstract

In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating the strains. By combining of the results of both techniques, we identified 40 genotypes. Molecular typing revealed a high genomic heterogeneity of the strains of F. solani studied.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
SD and UPGMA cluster analysis based on ERIC-PCR-produced patterns. First column, patient number; second column, isolate number; third column, ERIC type.
FIG. 2.
FIG. 2.
SD and UPGMA cluster analysis based on the combined AluI, CfoI, HaeIII, MboI, and NarI RFLP-produced patterns. First column, patient number; second column, isolate number; third column, PCR-RFLP type.
See this image and copyright information in PMC

References

    1. Bart-Delabesse, E., J. Sarfati, J. P. Debeaupuis, W. van Leeuwen, A. van Belkum, S. Bretagne, and J. P. Latge. 2001. Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates. J. Clin. Microbiol. 39:2683-2686. - PMC - PubMed
    1. Behrens-Baumann, W. 1999. Mycosis of the eye and its adnexa, p. 201. In W. Behrens-Baumann (ed.), Developments in ophthalmology, vol. 32. Karger, Basel, Switzerland.
    1. De Hoog, G. S., J. Guarro, J. Gené, and M. J. Figueras. 2000. Atlas of clinical fungi, p. 1126. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
    1. Guarro, J., M. Nucci, T. Akiti, and J. Gené. 2000. Mixed infection caused by two species of Fusarium in a human immunodeficiency virus-positive patient. J. Clin. Microbiol. 38:3460-3462. - PMC - PubMed
    1. Guarro, J., C. Rubio, J. Gené, J. Cano, J. Gil, R. Benito, M. J. Moranderia, and E. Míguez. 2003. Case of keratitis caused by an uncommon Fusarium species. J. Clin. Microbiol. 41:5823-5826. - PMC - PubMed

Publication types

LinkOut - more resources