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Comparative Study
. 2004 Oct;25(18-19):3270-6.
doi: 10.1002/elps.200406057.

Pyrimidine 5'-nucleotidase activities detected in real samples by means of micellar electrokinetic chromatography

Chiara Zanone  1 , Laurent R Chiarelli, Giovanna Valentini, Eleonora Perani, Laura Annovazzi, Simona Viglio, Paolo Iadarola
Affiliations
Comparative Study

Pyrimidine 5'-nucleotidase activities detected in real samples by means of micellar electrokinetic chromatography

Chiara Zanone et al. Electrophoresis. 2004 Oct.

Abstract

A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucleotidase and transferase activities of cytosolic 5'-nucleotidase III is presented. This electrophoretic approach was successfully applied to human erythrocyte lysates to monitor the enzyme activities indicated above, using either physiological substrate or the nucleoside analogue 3'-azido-3'-deoxythymidine as the phosphate acceptor.

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