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. 1992 Feb 1;111(1):135-9.
doi: 10.1016/0378-1119(92)90615-v.

The K2-type killer toxin- and immunity-encoding region from Saccharomyces cerevisiae: structure and expression in yeast

A Meskauskas  1 , D Citavicius
Affiliations

The K2-type killer toxin- and immunity-encoding region from Saccharomyces cerevisiae: structure and expression in yeast

A Meskauskas et al. Gene. .

Abstract

The cDNA copies of M2-1, the larger heat-cleavage product of M2 double-stranded (ds) RNA, have been synthesized, cloned, sequenced and expressed in yeast. This sequence, in combination with the known terminal sequence of M2-1 dsRNA, identifies a translation reading frame for a 362-amino-acid protein of 38.7 kDa, similar in size to the one of several protein species produced from M2-1 dsRNA in vitro translation. The expression of this cDNA clone in yeast confers both killer and immunity phenotypes.

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