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. 1992 Feb 1;111(1):143-4.
doi: 10.1016/0378-1119(92)90617-x.

A modified kanamycin-resistance cassette to facilitate two-codon insertion mutagenesis

W J Chen  1 , L Gross, K E Joho, W T McAllister
Affiliations

A modified kanamycin-resistance cassette to facilitate two-codon insertion mutagenesis

W J Chen et al. Gene. .

Abstract

A kanamycin-resistance (KmR) cassette was modified to allow its introduction as a blunt-ended DNA fragment into target plasmids. Subsequent excision of the KmR cassette with ApaI and religation of the plasmid leaves behind six base pairs (5'-GGGCCC) that result in the insertion of two amino acids in the target protein.

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