Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Nov;63(11):1478-82.
doi: 10.1136/ard.2003.017939.

High avidity anti-beta 2-glycoprotein I antibodies in patients with antiphospholipid syndrome

S Cucnik  1 , T Kveder, I Krizaj, B Rozman, B Bozic
Affiliations

High avidity anti-beta 2-glycoprotein I antibodies in patients with antiphospholipid syndrome

S Cucnik et al. Ann Rheum Dis. 2004 Nov.

Abstract

Objective: To evaluate avidity of IgG anti-beta 2-glycoprotein I antibodies (anti-beta2-GPI) in patients with antiphospholipid syndrome (APS) and systemic lupus erythematosus (SLE) in relation to thrombosis, and to demonstrate a possible affinity maturation of IgG anti-beta 2-GPI during the disease course.

Methods: 64 sera from 32 patients (18 with primary or secondary APS, 14 with SLE without APS) and their respective IgG fractions or affinity purified anti-beta 2-GPI were studied by anticardiolipin (aCL) and anti-beta 2-GPI enzyme linked immunosorbent assay and by chaotropic assay.

Results: Six, 12, and 14 patients had high, low, and heterogeneous avidity IgG anti-beta 2-GPI, respectively. In 12 patients an increase in antibody avidity was observed over a period of between four and 12 years. More patients with APS were in the high avidity than in the low avidity anti-beta 2-GPI group, while the opposite was observed for SLE alone (both p<0.05). The most common clinical feature among patients with high avidity anti-beta 2-GPI was thrombosis, mainly venous thrombosis (p<0.05 and p<0.02, respectively, v the low avidity anti-beta 2-GPI group).

Conclusions: Patients with APS with or without SLE may have anti-beta2-GPI of high, low, or heterogeneous avidity. High avidity anti-beta 2-GPI appear to be associated with thrombosis and APS, while in pure SLE low avidity anti-beta 2-GPI may prevail. Monitoring of avidity may help elucidate the role of anti-beta 2-GPI affinity maturation in the pathogenesis of APS.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The example of high, heterogeneous, and low avidity anti-ß2-glycoprotein I antibodies.
Figure 2
Figure 2
Avidity determination of anti-ß2-glycoprotein I antibodies in diluted serum. mOD: absorbance x1000.
Figure 3
Figure 3
Effect of matrix on the detection of high avidity anti-ß2-glycoprotein I antibodies. mOD: absorbance x1000.
Figure 4
Figure 4
The example of an affinity maturation of anti-ß2-glycoprotein I antibodies in paired samples from the same patient but different blood collections.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Br J Haematol. 2000 Apr;109(1):187-94 - PubMed
    1. J Immunol. 1999 Oct 15;163(8):4604-11 - PubMed
    1. Cancer Res. 2000 Dec 15;60(24):7008-13 - PubMed
    1. Curr Opin Rheumatol. 2001 Sep;13(5):377-82 - PubMed
    1. Clin Exp Rheumatol. 2002 Jan-Feb;20(1):101-8 - PubMed

Publication types