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. 2004 Oct;19(5):735-8.
doi: 10.3346/jkms.2004.19.5.735.

Detection rates of bacteria in chronic otitis media with effusion in children

Chul-Won Park  1 , Jang-Hee Han, Jin-Hyeok Jeong, Seok-Hyun Cho, Mi-Jung Kang, Kyung Tae, Seung-Hwan Lee
Affiliations

Detection rates of bacteria in chronic otitis media with effusion in children

Chul-Won Park et al. J Korean Med Sci. 2004 Oct.

Abstract

This study was performed to investigate polymerase chain reaction-based detection of bacterial DNA in middle ear fluid and assess the correlation between the PCR-positive rate with several factors associated with middle ear effusion. The purpose was to gain a further understanding of bacterial infection as a major cause of otitis media with effusion. Of the 278 specimens of middle ear fluid, 39 (14%) tested positive by ordinary culture. The overall detection rate of bacterial DNA using the PCR method was 36.7% for middle ear effusion, and bacterial DNA detection rates of Hemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis in the middle ear effusion were 29.1%, 4.7% and 10.8%, respectively. The bacterial DNA detection rate was higher in ears with a history of acute otitis media than those without the history. High detection rates were observed in patients younger than 48 months who have had a higher tendency to present with acute otitis media. We concluded that PCR is a more sensitive method for the detection of bacteria in middle ear effusion than ordinary culture, and acute otitis media is a major contributor to the pathogenesis of otitis media with effusion.

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Figures

Fig. 1
Fig. 1
Distribution of age of the subjected patients.
Fig. 2
Fig. 2
Detection of bacterial DNA fragments by polymerase chain reaction.
See this image and copyright information in PMC

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