Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Nov 15;10(22):3251-4.
doi: 10.3748/wjg.v10.i22.3251.

Detection of bcl-2 and bax expression and bcl-2/JH fusion gene in intrahepatic cholangiocarcinoma

Lin-Lang Guo  1 , Sha Xiao, Ying Guo
Affiliations

Detection of bcl-2 and bax expression and bcl-2/JH fusion gene in intrahepatic cholangiocarcinoma

Lin-Lang Guo et al. World J Gastroenterol. .

Abstract

Aim: To investigate the relationship between bcl-2 gene and its related protein bax and intrahepatic cholangiocellular carcinoma (CCC).

Methods: Semi-nested in situ PCR (SNISPCR) and immunohistochemistry were performed to detect bcl-2/JH fusion gene and bcl-2, bax protein expression in 29 cases of CCC.

Results: No bcl-2/JH fusion gene was found in all cases of CCC, 72.4% of 29 cases expressed bcl-2 protein. Bcl-2 protein expression was related to histopathological grades (P<0.05). There was no corresponding relationship between bcl-2/JH fusion gene formation and bcl-2 protein expression in CCC (P<0.05). Bax was expressed in 10.3% of 29 cases. The ratio of bcl-2 to bax in normal liver tissues (3.5 to 1) was different from that in tumor tissues (7.0 to 1).

Conclusion: It is suggested that bcl-2/JH fusion gene formation is not a frequent event and may not play an important role in the pathogenesis of CCC. However, aberrant ratio of bcl-2 to bax protein expression may be involved in the course of tumorigenesis of CCC. Abnormal bcl-2 protein expression may not be solely resulted from bcl-2/JH fusion gene.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Primary hepatic cholangiocarcinoma. Brown-yellow staining in cytoplasm of carcinoma cells for bcl-2 protein (arrowhead) Immunohistochemistry staining × 200.
Figure 2
Figure 2
Normal liver. Brown-yellow staining in cytoplasm of small bile duct epithelial cells for bcl-2 protein (arrowhead) Immunohistochemistry staining × 200.
Figure 3
Figure 3
Primary hepatic cholangiocarcinoma. Brown-yellow staining in cytoplasm of carcinoma cells for bax protein (arrowhead) Immunohistochemistry staining × 200.
Figure 4
Figure 4
Normal liver. Brown-yellow staining in cytoplasm of small bile duct epithelial cells for bax protein (arrowhead) Immunohistochemistry staining × 200.
Figure 5
Figure 5
Non-Hodgkin's lymphoma. Purple-blue deposition in nuclear of lympoma cells (positive for mbr, arrowhead). In situ PCR × 400.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Tsujimoto Y, Finger LR, Yunis J, Nowell PC, Croce CM. Cloning of the chromosome breakpoint of neoplastic B cells with the t(14; 18) chromosome translocation. Science. 1984;226:1097–1099. - PubMed
    1. Weiss LM, Warnke RA, Sklar J, Cleary ML. Molecular analysis of the t(14; 18) chromosomal translocation in malignant lymphomas. N Engl J Med. 1987;317:1185–1189. - PubMed
    1. Bakhshi A, Jensen JP, Goldman P, Wright JJ, McBride OW, Epstein AL, Korsmeyer SJ. Cloning the chromosomal breakpoint of t(14; 18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 1985;41:899–906. - PubMed
    1. Aisenberg AC, Wilkes BM, Jacobson JO. The bcl-2 gene is rearranged in many diffuse B-cell lymphomas. Blood. 1988;71:969–972. - PubMed
    1. McDonnell TJ, Troncoso P, Brisbay SM, Logothetis C, Chung LW, Hsieh JT, Tu SM, Campbell ML. Expression of the protooncogene bcl-2 in the prostate and its association with emergence of androgen-independent prostate cancer. Cancer Res. 1992;52:6940–6944. - PubMed

MeSH terms

Substances