Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Aug;10(8):1482-5.
doi: 10.3201/eid1008.030784.

Human Escherichia coli O157:H7 genetic marker in isolates of bovine origin

Jeffrey T Lejeune  1 , Stephen T Abedon, Kaori Takemura, Nicholas P Christie, Srinand Sreevatsan
Affiliations

Human Escherichia coli O157:H7 genetic marker in isolates of bovine origin

Jeffrey T Lejeune et al. Emerg Infect Dis. 2004 Aug.

Abstract

The antiterminator Q gene of bacteriophage 933W (Q933) was identified upstream of the stx2 gene in 90% of human disease-origin Escherichia coli O157:H7 isolates and in 44.5% of bovine isolates. Shiga toxin production was higher in Q933-positive isolates than Q933-negative isolates. This genetic marker may provide a useful molecular tool for epidemiologic studies.

PubMed Disclaimer

Figures

Figure
Figure
Ethidium bromide–stained gel of the amplification products obtained from Q933-595 and Q21-595 polymerase chain reactions. aEDL933, human isolate (ATCC43895). Obtained from the STEC Center, Michigan State University. bFAHRP88, isolated from Ohio dairy cow. cFAHRP39, human isolate (E29962) (12).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Karmali MA, Arbus GS, Petric M, Patrick ML, Roscoe M, Shaw J, et al. Hospital-acquired Escherichia coli O157:H7 associated haemolytic uraemic syndrome in a nurse [letter]. Lancet. 1988;1:526. 10.1016/S0140-6736(88)91310-4 - DOI - PubMed
    1. O'Loughlin EV, Robins-Browne RM. Effect of Shiga toxin and Shiga-like toxins on eukaryotic cells. Microbes Infect. 2001;3:493–507. 10.1016/S1286-4579(01)01405-8 - DOI - PubMed
    1. Kimura N, Watanabe M, Komatsubara A. [Verotoxin producing ability of verotoxin-producing Escherichia coli strains isolated from fecal specimens of healthy persons is lower than that of patients]. Kansenshogaku Zasshi. 2000;74:849–51. - PubMed
    1. Pradel N, Boukhors K, Bertin Y, Forestier C, Martin C, Livrelli V. Heterogeneity of Shiga toxin-producing Escherichia coli strains isolated from hemolytic-uremic syndrome patients, cattle, and food samples in central France. Appl Environ Microbiol. 2001;67:2460–8. 10.1128/AEM.67.6.2460-2468.2001 - DOI - PMC - PubMed
    1. Wagner PL, Neely MN, Zhang X, Acheson DW, Waldor MK, Friedman DI. Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strain. J Bacteriol. 2001;183:2081–5. 10.1128/JB.183.6.2081-2085.2001 - DOI - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources