Proteomics in platelet science

Abstract

Proteomics, the rapid large-scale analysis of cellular proteins, is becoming an important tool in the study of human platelet biology. A number of investigators have used proteomics techniques to study platelets including two-dimensional gel electrophoresis, protein separation, and mass spectrometry protein identification. Most efforts have focused on: 1) cataloging the spectrum of proteins that comprise the normal platelet "proteome", 2) characterizing proteins released from activated platelets, and 3) identifying phosphoproteins generated upon platelet stimulation. The incorporation of modern proteomics technology to platelet studies will further our understanding of platelet activation, adhesion, and aggregation at the protein level. Moreover, proteomics offers hope that novel biomarkers can be discovered that diagnose inherited and/or acquired platelet disorders, or predict the susceptibility of such individuals to bleeding or thrombosis. Finally, proteomics could lead to identification of novel agents that have therapeutic value. This review discusses the current status and future opportunities of proteomics technologies in platelet science.