Mutation induction by 5-fluorodeoxyuridine in synchronous Chinese hamster cells
- PMID: 154970
Item in Clipboard
Mutation induction by 5-fluorodeoxyuridine in synchronous Chinese hamster cells
Cancer Res.
1979 Mar.
Abstract
Chinese hamster ovary cells synchronized by mitotic detachment were treated with 5-fluorodeoxyuridine (FdUrd), 0.2 micrograms/ml, at various times in the cell cycle. FdUrd treatment in the early S period induced resistance to 6-thioguanine. Treatment of asynchronous cells with FdUrd caused little increase in 6-thioguanine resistance over the spontaneous frequency. Mitotic selection thus enhances the probability of a cell being in the portion of S period receptive to mutation. These observations suggest that FdUrd treatment leads to mutagenesis at the growing points of DNA replication.
Similar articles
-
X-ray induction of 8-azaguanine-resistant mutants in synchronous Chinese hamster ovary cells.Radiat Res. 1980 Jul;83(1):146-61. Radiat Res. 1980. PMID: 7394161 No abstract available.
-
Occurrence of induced 6-thioguanine-resistant colonies in synchronized V79 cells after treatment with ftorafur. Effects of the S9 fraction.Neoplasma. 1984;31(3):339-46. Neoplasma. 1984. PMID: 6234472
-
Action of FdUrD and dCyd on the incorporation of BrdUrd in chinese hamster somatic cell DNA and the isolation of auxotrophic mutants.J Cell Physiol. 1977 Feb;90(2):321-7. doi: 10.1002/jcp.1040900217. J Cell Physiol. 1977. PMID: 138686
-
Ethylnitrosourea-induced mutagenesis in asynchronous and synchronous Chinese hamster ovary cells.Mutat Res. 1980 Jan;69(1):127-37. doi: 10.1016/0027-5107(80)90182-7. Mutat Res. 1980. PMID: 6987492
-
Deoxyribonucleoside-induced selective modulation of cytotoxicity and mutagenesis.Basic Life Sci. 1985;31:313-34. doi: 10.1007/978-1-4613-2449-2_19. Basic Life Sci. 1985. PMID: 3158302 Review.
Cited by
-
DNA mismatch repair (MMR)-dependent 5-fluorouracil cytotoxicity and the potential for new therapeutic targets.Br J Pharmacol. 2009 Oct;158(3):679-92. doi: 10.1111/j.1476-5381.2009.00423.x. Epub 2009 Sep 23. Br J Pharmacol. 2009. PMID: 19775280 Free PMC article. Review.
-
Differential cell cycle phase specificity for neoplastic transformation and mutation to ouabain resistance induced by N-methyl-N'-nitro-N-nitrosoguanidine in synchronized C3H10T 1/2 C18 cells.Proc Natl Acad Sci U S A. 1982 Jul;79(14):4342-6. doi: 10.1073/pnas.79.14.4342. Proc Natl Acad Sci U S A. 1982. PMID: 6956864 Free PMC article.
-
Cell cycle-specific mutagenesis at the hypoxanthine phosphoribosyltransferase locus in adult rat liver epithelial cells.Proc Natl Acad Sci U S A. 1980 Dec;77(12):7377-9. doi: 10.1073/pnas.77.12.7377. Proc Natl Acad Sci U S A. 1980. PMID: 6938982 Free PMC article.