Granzyme B; the chalk-mark of a cytotoxic lymphocyte

Nigel J Waterhouse¹, Karin A Sedelies, Chris Jp Clarke

Affiliations

PMID: 15500699
PMCID: PMC528853
DOI: 10.1186/1479-5876-2-36

Editorial

Granzyme B; the chalk-mark of a cytotoxic lymphocyte

Nigel J Waterhouse et al. J Transl Med. 2004.

. 2004 Oct 25;2(1):36.

doi: 10.1186/1479-5876-2-36.

Authors

Nigel J Waterhouse¹, Karin A Sedelies, Chris Jp Clarke

Affiliation

¹ Cancer Immunology Program, Peter MacCallum Cancer Centre, Locked Bag 1, A'Beckett Street, Melbourne, 8006, Australia. nigel.waterhouse@petermac.org.

PMID: 15500699
PMCID: PMC528853
DOI: 10.1186/1479-5876-2-36

Abstract

During cytotoxic lymphocyte (CL) mediated killing of target cells, granzyme B is released from the CL into the immune synapse. Recent studies have found that ELISPOT-detection of granzyme B correlated well with conventional assays for CL mediated killing. In this way, the released granzyme B can be used to mark the spot where a target cell was murdered. We discuss the benefits and potential limitations of using this assay to measure CL mediated killing of target cells.

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References

1. Waterhouse NJ, Trapani JA. CTL: Caspases Terminate Life, but that's not the whole story. Tissue Antigens. 2002;59:175–183. doi: 10.1034/j.1399-0039.2002.590301.x. - DOI - PubMed
1. Waterhouse NJ, Clarke CJ, Sedelies KA, Teng MW, Trapani JA. Cytotoxic lymphocytes; instigators of dramatic target cell death. Biochem Pharmacol. 2004;68:1033–1040. doi: 10.1016/j.bcp.2004.05.043. - DOI - PubMed
1. Roden MM, Lee KH, Panelli MC, Marincola FM. A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology. J Immunol Methods. 1999;226:29–41. doi: 10.1016/S0022-1759(99)00039-3. - DOI - PubMed
1. Burrows SR, Suhrbier A, Khanna R, Moss DJ. Rapid visual assay of cytotoxic T-cell specificity utilizing synthetic peptide induced T-cell-T-cell killing. Immunology. 1992;76:174–175. - PMC - PubMed
1. Goldberg JE, Sherwood SW, Clayberger C. A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry. J Immunol Methods. 1999;224:1–9. doi: 10.1016/S0022-1759(98)00038-6. - DOI - PubMed

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Granzyme B; the chalk-mark of a cytotoxic lymphocyte

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Granzyme B; the chalk-mark of a cytotoxic lymphocyte

Authors

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Abstract

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