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. 2004 Oct;95(10):822-7.
doi: 10.1111/j.1349-7006.2004.tb02188.x.

Overexpression of the Wilms' tumor gene W T1 in primary astrocytic tumors

Yusuke Oji  1 , Tsuyoshi Suzuki, Yoko Nakano, Motohiko Maruno, Shin-Ichi Nakatsuka, Tanyarat Jomgeow, Sakie Abeno, Naoya Tatsumi, Asumi Yokota, Sayaka Aoyagi, Tsutomu Nakazawa, Ken Ito, Keisuke Kanato, Toshiaki Shirakata, Sumiyuki Nishida, Naoki Hosen, Manabu Kawakami, Akihiro Tsuboi, Yoshihiro Oka, Katsuyuki Aozasa, Toshiki Yoshimine, Haruo Sugiyama
Affiliations

Overexpression of the Wilms' tumor gene W T1 in primary astrocytic tumors

Yusuke Oji et al. Cancer Sci. 2004 Oct.

Abstract

Expression of the Wilms' tumor gene W T1 in primary astrocytic tumors was examined using a quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) or immunohistochemistry. Real-time RT-PCR showed that W T1 mRNA was expressed at various levels in all of the 25 astrocytic tumors examined. Immunohistochemical analysis showed that W T1 protein was expressed in 5 of 6 low-grade astrocytic tumors (grade I-II) and all of 18 high-grade ones (grade III-IV), and that expression levels of W T1 protein in high-grade tumors were significantly higher than those in low-grade ones. W T1 protein was not detected in the normal glial cells contained in the tumor specimens. Furthermore, treatment with W T1 antisense oligomers specifically inhibited growth of glioblastoma cell lines, U87-MG, A172, and T-98G. These results may indicate that the W T1 gene plays an important role in tumorigenesis of primary astrocytic tumors.

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