Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium

Abstract

Influenza virus neuraminidase (NA) plays an essential role in release and spread of progeny virions, following the intracellular viral replication cycle. To test whether NA could also facilitate virus entry into cell, we infected cultures of human airway epithelium with human and avian influenza viruses in the presence of the NA inhibitor oseltamivir carboxylate. Twenty- to 500-fold less cells became infected in drug-treated versus nontreated cultures (P < 0.0001) 7 h after virus application, indicating that the drug suppressed the initiation of infection. These data demonstrate that viral NA plays a role early in infection, and they provide further rationale for the prophylactic use of NA inhibitors.

FIG. 1.

Cross-section of HTBE culture on a membrane support. Shown are immunostaining for cilia (black), periodic acid-Schiff′s reaction for goblet cell mucin (magenta), and counterstaining with hematoxylin. Bar = 20 μm.

FIG. 2.

En face view of HTBE cultures infected with human influenza virus A/Memphis/14/96 in the presence of OC (b) and in its absence (a). Shown is immunostaining for virus antigen (black). Bar = 20 μm.