Review
[False positive and false negative reaction results based on an amplification of nucleic acids in infection diagnoses. Reasons and implications]
[Article in
Polish]
Affiliations
- PMID: 15517815
Item in Clipboard
Review
[False positive and false negative reaction results based on an amplification of nucleic acids in infection diagnoses. Reasons and implications]
[Article in
Polish]
Przegl Epidemiol.
2004.
Abstract
The paper discusses the most frequent causes of false positive and false negative results of reactions based on amplification of nucleic acids. All stages of the method are described beginning with sample collection up to the finishing of visualization of amplification product. The main aim objective of the publication is to draw doctors' attention to the necessity to interpret the result with criticism. We attempted also to demonstrate how to avoid false results.
Similar articles
-
[Gene amplification for the diagnosis of HIV infection].Rev Roum Virol. 1992 Jul-Dec;43(3-4):207-20. Rev Roum Virol. 1992. PMID: 1308433 Review. French. No abstract available.
-
Equivalent amplification of intrinsically variable nucleic acid sequences by multiple-primer-induced overlapping amplification assay: applications for universal detection and quantitation.Nat Med. 1999 Sep;5(9):1081-5. doi: 10.1038/12520. Nat Med. 1999. PMID: 10470090 No abstract available.
-
Chlamydia trachomatis RNA in the environment: is there potential for false-positive nucleic acid amplification test results?Sex Transm Infect. 2008 Apr;84(2):107-10. doi: 10.1136/sti.2007.027862. Epub 2007 Oct 30. Sex Transm Infect. 2008. PMID: 17971374
-
DNA typing by microbead arrays and PCR-SSP: apparent false-negative or -positive hybridization or amplification signals disclose new HLA-B and -DRB1 alleles.Tissue Antigens. 2008 Mar;71(3):238-41. doi: 10.1111/j.1399-0039.2007.00993.x. Epub 2008 Jan 7. Tissue Antigens. 2008. PMID: 18179646
-
[RNA amplification from low numbers of bacteria in human blood to solve low sensitivity problem of conventional PCR amplification].Rinsho Byori. 2006 Oct;54(10):1055-8. Rinsho Byori. 2006. PMID: 17133994 Review. Japanese.