doi: 10.1021/ja040175z.
A genetically encoded photocaged amino acid
Affiliations
- PMID: 15521721
- DOI: 10.1021/ja040175z
Item in Clipboard
A genetically encoded photocaged amino acid
J Am Chem Soc.
.
Abstract
We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.
Similar articles
-
Adaptation of an orthogonal archaeal leucyl-tRNA and synthetase pair for four-base, amber, and opal suppression.Biochemistry. 2003 Aug 19;42(32):9598-608. doi: 10.1021/bi034550w. Biochemistry. 2003. PMID: 12911301
-
A promiscuous aminoacyl-tRNA synthetase that incorporates cysteine, methionine, and alanine homologs into proteins.Bioorg Med Chem Lett. 2008 Nov 15;18(22):6004-6. doi: 10.1016/j.bmcl.2008.09.050. Epub 2008 Sep 17. Bioorg Med Chem Lett. 2008. PMID: 18845434
-
Discrimination of tRNA(Leu) isoacceptors by the mutants of Escherichia coli leucyl-tRNA synthetase in editing.Biochemistry. 2002 Aug 27;41(34):10623-8. doi: 10.1021/bi026000o. Biochemistry. 2002. PMID: 12186547
-
Recognition of human mitochondrial tRNALeu(UUR) by its cognate leucyl-tRNA synthetase.J Mol Biol. 2004 May 21;339(1):17-29. doi: 10.1016/j.jmb.2004.03.066. J Mol Biol. 2004. PMID: 15123417
-
Discrimination of tRNALeu isoacceptors by the insertion mutant of Escherichia coli leucyl-tRNA synthetase.Biochemistry. 1999 Jul 13;38(28):9084-8. doi: 10.1021/bi9901984. Biochemistry. 1999. PMID: 10413482
Cited by
-
Cell-Permeable Stimuli-Responsive Ubiquitin Probe for Time-Resolved Monitoring of Substrate Ubiquitination in Live Cells.JACS Au. 2023 Oct 3;3(10):2873-2882. doi: 10.1021/jacsau.3c00421. eCollection 2023 Oct 23. JACS Au. 2023. PMID: 37885572 Free PMC article.
-
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells.J Vis Exp. 2018 Apr 9;(134):57069. doi: 10.3791/57069. J Vis Exp. 2018. PMID: 29683449 Free PMC article.
-
Structural Robustness Affects the Engineerability of Aminoacyl-tRNA Synthetases for Genetic Code Expansion.Biochemistry. 2021 Feb 23;60(7):489-493. doi: 10.1021/acs.biochem.1c00056. Epub 2021 Feb 9. Biochemistry. 2021. PMID: 33560840 Free PMC article.
-
Automated orthogonal tRNA generation.Nat Chem Biol. 2025 May;21(5):657-667. doi: 10.1038/s41589-024-01782-3. Epub 2024 Dec 20. Nat Chem Biol. 2025. PMID: 39706894 Free PMC article.
-
Light activation as a method of regulating and studying gene expression.Curr Opin Chem Biol. 2009 Dec;13(5-6):678-86. doi: 10.1016/j.cbpa.2009.09.026. Epub 2009 Oct 24. Curr Opin Chem Biol. 2009. PMID: 19857985 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
