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. 2004 Nov 10;126(44):14306-7.
doi: 10.1021/ja040175z.

A genetically encoded photocaged amino acid

Ning Wu  1 , Alexander Deiters, T Ashton Cropp, David King, Peter G Schultz
Affiliations

A genetically encoded photocaged amino acid

Ning Wu et al. J Am Chem Soc. .

Abstract

We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.

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