Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2004 Nov;42(11):5139-45.
doi: 10.1128/JCM.42.11.5139-5145.2004.

Nocardia kruczakiae sp. nov., a pathogen in immunocompromised patients and a member of the "N. nova complex"

Patricia S Conville  1 , June M Brown, Arnold G Steigerwalt, Judy W Lee, Victoria L Anderson, Joel T Fishbain, Steven M Holland, Frank G Witebsky
Affiliations
Case Reports

Nocardia kruczakiae sp. nov., a pathogen in immunocompromised patients and a member of the "N. nova complex"

Patricia S Conville et al. J Clin Microbiol. 2004 Nov.

Abstract

Molecular methodologies have become useful techniques for the identification of pathogenic Nocardia species and for the recognition of novel species that are capable of causing human disease. Two isolates recovered from immunocompromised patients were characterized as Nocardia nova by biochemical and susceptibility testing results. The restriction fragment length polymorphism (RFLP) patterns obtained by restriction endonuclease analysis (REA) of an amplified portion of the heat shock protein gene were identical to those obtained with the type strain of N. nova. REA of an amplified portion of the 16S rRNA gene showed RFLP patterns that were unlike those obtained for the type strain of N. nova but that were similar to those obtained for the type strains of N. africana and N. veterana. Subsequent sequencing of a portion of the 16S rRNA gene produced identical results for the two patient isolates. Sequence analysis of 1,352-bp portions of the 16S rRNA gene indicated that these isolates were 99.8% similar to the recently described species N. veterana but were only 99.3, 98.1, and 98.1% similar to the type strains of N. africana, N. nova, and N. vaccinii, respectively. DNA-DNA hybridization studies confirmed that the two patient isolates belonged to the same species but were not closely related to N. africana, N. nova, N. vaccinii, or N. veterana. The patient isolates have been designated N. kruczakiae sp. nov. Because N. africana, N. veterana, and the new species are not readily differentiated from N. nova by phenotypic methods alone, the designation "N. nova complex" can be used to designate isolates such as these that phenotypically resemble N. nova but that have not been definitively characterized by 16S rRNA gene sequencing or DNA-DNA hybridization.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Phylogenetic tree of type strains of Nocardia species and patient isolates obtained by using the ClustalV algorithm of MegAlign software (DNASTAR, Inc.). Boldface indicates N. nova and organisms phenotypically resembling it.
See this image and copyright information in PMC

References

    1. Brenner, D. J., F. W. Hickman-Brenner, J. V. Lee, A. G. Steigerwalt, G. R. Fanning, D. G. Hollis, J. J. Farmer III, R. E. Weaver, S. W. Joseph, and R. Seidler. 1983. Vibrio furnissii (formerly aerogenic biogroup of Vibrio fluvialis), a new species isolated from human feces and the environment. J. Clin. Microbiol. 18:816-824. - PMC - PubMed
    1. Conville, P. S., J. M. Brown, A. G. Steigerwalt, J. W. Lee, D. E. Byrer, V. L. Anderson, S. E. Dorman, S. M. Holland, B. Cahill, K. C. Carroll, and F. G. Witebsky. 2003. Nocardia veterana as a pathogen in North American patients. J. Clin. Microbiol. 41:2560-2568. - PMC - PubMed
    1. Conville, P. S., S. H. Fischer, C. P. Cartwright, and F. G. Witebsky. 2000. Identification of Nocardia species by restriction endonuclease analysis of an amplified portion of the 16S rRNA gene. J. Clin. Microbiol. 38:158-164. - PMC - PubMed
    1. Dorman, S. E., S. V. Guide, P. S. Conville, E. S. DeCarlo, H. L. Malech, J. I. Gallin, F. G. Witebsky, and S. M. Holland. 2002. Nocardia infection in chronic granulomatous disease. Clin. Infect. Dis. 35:390-394. - PubMed
    1. Gürtler, V., R. Smith, B. C. Mayall, G. Pötter-Reinemann, E. Stackebrandt, and R. M. Kroppenstedt. 2001. Nocardia veterana sp. nov., isolated from human bronchial lavage. Int. J. Syst. Evol. Microbiol. 51:933-936. - PubMed

Publication types

MeSH terms

Associated data

LinkOut - more resources