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. 1992 Jan;420(1):115-7.
doi: 10.1007/BF00378652.

The effects of thapsigargin on [Ca2+]i in isolated rat mesenteric artery vascular smooth muscle cells

I Baró  1 , D A Eisner
Affiliations

The effects of thapsigargin on [Ca2+]i in isolated rat mesenteric artery vascular smooth muscle cells

I Baró et al. Pflugers Arch. 1992 Jan.

Abstract

The effects of thapsigargin were studied on single cells isolated from side branches of the rat mesenteric artery. Thapsigargin (150 nM) produced a transient increase of [Ca2+]i. This transient rise of [Ca2+]i was unaffected by removing external Ca2+ ions. This suggests that thapsigargin is releasing Ca2+ ions from an intracellular store. In the absence of thapsigargin both noradrenaline and caffeine also produced a transient increase of [Ca2+]i. These increases were abolished by prior exposure to thapsigargin. Correspondingly, the effects of thapsigargin were abolished by prior exposure to caffeine. These results show that thapsigargin releases Ca2+ from the noradrenaline and caffeine-sensitive stores.

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