Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Jan;420(1):121-3.
doi: 10.1007/BF00378654.

Single cell assay of exocytosis from pancreatic islet B cells

K D Gillis  1 , S Misler
Affiliations

Single cell assay of exocytosis from pancreatic islet B cells

K D Gillis et al. Pflugers Arch. 1992 Jan.

Abstract

We have measured changes in membrane capacitance (delta Cm) in pancreatic B cells as a single cell assay of insulin secretion. Evidence that depolarization evoked delta Cm reflects exocytosis includes it's voltage, temperature and Ca2+ o dependence. Decreases in Cm, presumably reflecting endocytosis, occur on a variable time scale. Two features that make B cells unique among excitable cells are the large magnitude of delta Cm, when normalized to Ca2+ current, and the rapid "fatigue" of the response for even minimal stimulation, perhaps due to the depletion of a readily releasable pool of vesicles.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Gen Physiol. 1988 May;91(5):617-39 - PubMed
    1. Methods Enzymol. 1983;98:182-200 - PubMed
    1. J Gen Physiol. 1988 Aug;92(2):145-59 - PubMed
    1. EMBO J. 1990 Jan;9(1):53-60 - PubMed
    1. Pflugers Arch. 1991 Jul;418(6):611-3 - PubMed

Publication types

LinkOut - more resources