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. 1992 Jan;420(1):121-3.
doi: 10.1007/BF00378654.

Single cell assay of exocytosis from pancreatic islet B cells

K D Gillis  1 , S Misler
Affiliations

Single cell assay of exocytosis from pancreatic islet B cells

K D Gillis et al. Pflugers Arch. 1992 Jan.

Abstract

We have measured changes in membrane capacitance (delta Cm) in pancreatic B cells as a single cell assay of insulin secretion. Evidence that depolarization evoked delta Cm reflects exocytosis includes it's voltage, temperature and Ca2+ o dependence. Decreases in Cm, presumably reflecting endocytosis, occur on a variable time scale. Two features that make B cells unique among excitable cells are the large magnitude of delta Cm, when normalized to Ca2+ current, and the rapid "fatigue" of the response for even minimal stimulation, perhaps due to the depletion of a readily releasable pool of vesicles.

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