Comparative studies on intestine ultrastructure of third-stage larvae and adults of Cystidicoloides ephemeridarum (Nematoda, Cystidicolidae)

Abstract

The intestinal epithelium of third-stage larvae and adults of Cystidicoloides ephemeridarum from haemocoel of mayflies and stomach of brown trout was studied by electron microscopy and cytochemistry. In section, the intestine of both stages is composed of a single layer of about ten undifferentiated intestinal cells in a ring. A labyrinth of deep invaginations is present in the basal region of each cell. The apical surface is modified into well developed, regularly arranged microvilli. These, together with numerous organelles engaged in metabolism and a well defined gut lumen filled with unidentifiable material suggest that the intestine may function in digestion and absorption during both stages. The adults seem to feed upon the semifluid content of the stomach of brown trout. Fortuitous oral infection with undetermined bacteria in vitro led to degenerative changes in the intestinal tissue and probably caused death of the infected specimens. Up to 75% of the cell volume in the L(3) is occupied by glycogen deposits. In the adults, a minor portion of glycogen, together with lipid droplets, has been observed. The adults are considered to rely more on aerobic metabolism, whereas anaerobic metabolism (glycolysis) may prevail in L(3).