[Using of 4-thiouridine and 4-thiothymidine for pyrimidine nucleoside phosphorylase studing]
[Article in
Russian]
- PMID: 15554192
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[Using of 4-thiouridine and 4-thiothymidine for pyrimidine nucleoside phosphorylase studing]
[Article in
Russian]
Mol Biol (Mosk).
2004 Sep-Oct.
Abstract
4-Thiouridine and 4-thiothymidine were developed as efficient substrates for spectrophotometric determination of uridine phosphorylase and thymidine phosphorylase activity. 4-Thiouridine has maximum absorbance at 330 nm (pH 7.5). The change in extinction coefficient for 4-thiouridine/4-thiouracil, deltaepsilon is 3000 M(-1) x cm(-1). It appeared that 4-thiouridine is a good substrate for uridine phosphorylase with Michaelis-Menten constant 130 microM and kcat 49 s(-1). In the case of 4-thiothymidine/4-thiothymine deltaepsilon is even larger: 5000 M(-1) x cm(-1) at 336 nm.
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