Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2004 Nov;37(5):835-9.

S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells

Affiliations
  • PMID: 15560139
Comparative Study

S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells

Jennifer S Hackbarth et al. Biotechniques. 2004 Nov.

Abstract

Epitope tags are widely used in cell biology and biochemistry research. The S-peptide/S-protein interaction has previously been utilized to purify polypeptides expressed in bacteria. We have now re-engineered the S-peptide/S-protein system to allow isolation of S-peptide-tagged polypeptides and their binding partners from eukaryotic cells with S-protein-agarose. In addition, two anti-S-peptide monoclonal antibodies have been generated for analysis of expression and subcellular localization of S-peptide-tagged polypeptides. These reagents make the S-peptide/S-protein system an attractive alternative to currently available epitope tagging methods.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources