Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Dec;48(12):4843-7.
doi: 10.1128/AAC.48.12.4843-4847.2004.

Distribution of antibiotic MICs for Helicobacter pylori strains over a 16-year period in patients from Seoul, South Korea

Jung Mogg Kim  1 , Joo Sung Kim, Hyun Chae Jung, Nayoung Kim, Young-Jeon Kim, In Sung Song
Affiliations

Distribution of antibiotic MICs for Helicobacter pylori strains over a 16-year period in patients from Seoul, South Korea

Jung Mogg Kim et al. Antimicrob Agents Chemother. 2004 Dec.

Abstract

Recently, the development of antibiotic resistance emerged as a significant clinical problem in the eradication of Helicobacter pylori. We investigated the MICs of antibiotics for 135 H. pylori isolates from adults in Seoul, South Korea, over the past 16 years. The MICs of amoxicillin, clarithromycin, metronidazole, tetracycline, azithromycin, and ciprofloxacin increased from 1987 to 2003. Rates of primary resistance to clarithromycin increased from 2.8% in 1994 to 13.8% in 2003. The A2144G mutation was frequently observed in the 23S rRNA gene in clarithromycin-resistant isolates. The increase in resistance to clarithromycin seems to result in a decrease in eradication efficacy for H. pylori. These results suggest that the MICs of several antibiotics for H. pylori have increased over the past 16 years in Seoul.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Distributions of antibiotic MICs for H. pylori over a 16-year period. Thirty-four strains were examined in 1987, 36 strains were examined in 1994, and 65 strains were examined in 2003. The color key in panel A applies to all panels. MICs were determined by use of the agar dilution method. MICs of amoxicillin (A), clarithromycin (B), metronidazole (C), tetracycline (D), azithromycin (E), and ciprofloxacin (F) are shown.
FIG. 2.
FIG. 2.
Restriction endonuclease analysis of 23S rRNA amplicons. (A) Digestion with BsaI; (B) digestion with BbsI. The A2144G mutation was found in lanes 5 to 9 and in lanes 11 and 12 but not in lanes 3, 4, and 10. Note that the A2143G mutation detected by digestion with BbsI was not detected in any of the strains studied. Lanes 3, 4, and 10 revealed the T2183C mutation, as assessed by DNA sequencing. Lane M, DNA size markers (indicated to the left of the gels in base pairs); lane 1, H. pylori ATCC 43504; lane 2, Escherichia coli DNA; lanes 3 to 12, clarithromycin-resistant H. pylori strains. Clarithromycin MICs are 16 (lane 3), 32 (lane 4), 32 (lane 5), 32 (lane 6), 32 (lane 7), 32 (lane 8), 16 (lane 9), 16 (lane 10), and 64 (lane 11) μg/ml.
See this image and copyright information in PMC

References

    1. Adamek, R. J., S. Suerbaum, B. Pfaffenbach, and W. Opferkuch. 1998. Primary and acquired Helicobacter pylori resistance to clarithromycin, metronidazole, and amoxicillin—influence on treatment outcome. Am. J. Gastroenterol. 93:386-389. - PubMed
    1. Boyanova, L., R. Koumanova, G. Gergova, M. Popova, I. Mitov, Y. Kovacheva, S. Derejian, N. Katsarov, R. Nikolov, and Z. Krastev. 2002. Prevalence of resistant Helicobacter pylori isolates in Bulgarian children. J. Med. Microbiol. 51:786-790. - PubMed
    1. Cabrita, J., M. Oleastro, R. Matos, A. Manhente, J. Cabral, R. Barros, A. I. Lopes, P. Ramalho, B. C. Neves, and A. S. Guerreiro. 2000. Features and trends in Helicobacter pylori antibiotic resistance in Lisbon area, Portugal (1990-1999). J. Antimicrob. Chemother. 46:1029-1031. - PubMed
    1. Glupczynski, Y., F. Megraud, M. Lopez-Brea, and L. P. Andersen. 2001. European multicentre survey of in vitro antimicrobial resistance in Helicobacter pylori. Eur. J. Clin. Microbiol. Infect. Dis. 20:820-823. - PubMed
    1. Graham, D. Y. 1998. Antibiotic resistance in Helicobacter pylori: implications for therapy. Gastroenterology 115:1272-1277. - PubMed

MeSH terms