Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Oct;23(8-9):1281-5.
doi: 10.1081/NCN-200027547.

Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells

M Löffler  1 , A Klein, M Hayek-Ouassini, W Knecht, L Konrad
Affiliations

Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells

M Löffler et al. Nucleosides Nucleotides Nucleic Acids. 2004 Oct.

Abstract

To follow the expression of the fourth enzyme of pyrimidine de novo synthesis dihydroorotate dehydrogenase (DHODH) in cells and tissues, we studied the DHODH mRNA expression by means of RT-PCR in rat tissues. Rabbit polyclonal anti-DHODH immunoglobulins were applied for immunochemical quantification of the enzyme protein by Western blotting. In mouse B-lymphocytes, which were adapted to tolerate up to a 50-fold concentration of the DHODH inhibitor leflunomide, a 20 fold protein overexpression was measured. Southern blotting indicated DHODH gene amplification.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources