Comparison of QTL controlling seedling vigour under different temperature conditions using recombinant inbred lines in rice (Oryza sativa)
- PMID: 15574482
- PMCID: PMC4246786
- DOI: 10.1093/aob/mci039
Comparison of QTL controlling seedling vigour under different temperature conditions using recombinant inbred lines in rice (Oryza sativa)
Abstract
Background and aims: Seedling vigour is one of the major determinants for stable stand establishment in rice (Oryza sativa), especially in a direct seeding cropping system. The objectives of this study were to identify superior alleles with consistent effects on seedling vigour across different temperature conditions and to investigate genotype x environmental temperature interactions for seedling vigour QTL.
Methods: A set of 282 F13 recombinant inbred lines (RILs) derived from a rice cross were assessed for four seedling vigour traits at three temperatures (25 degrees C, 20 degrees C and 15 degrees C). Using a linkage map with 198 marker loci, the main-effect QTL for the traits were mapped by composite interval mapping.
Key results: A total of 34 QTL for the four seedling vigour traits were identified. Of these QTL, the majority (82%) were clustered within five genomic regions, designated as QTL qSV-3-1, qSV-3-2, qSV-5, qSV-8-1 and qSV-8-2. All of these five QTL had small individual effects on the traits, explaining 3.1-15.8 % of the phenotypic variation with a mean of 7.3 %. QTL qSV-3-1, qSV-3-2 and qSV-8-1 showed almost consistent effects on the traits across all three temperatures while qSV-5 and qSV-8-2 had effects mainly at the 'normal' temperatures of 20 degrees C and 25 degrees C. Among the five QTL identified, all and four showed additive effects on shoot length and germination rate, respectively. The contributions of these five QTL to shoot length and germination rate were also much larger than those to the other two traits.
Conclusions: A few of genomic regions (or QTL) were identified as showing effects on seedling vigour. For these QTL, significant genotype x environmental temperature interactions were found and these interactions appeared to be QTL-specific. Among the four seedling vigour traits measured, shoot length and germination rate could be used as relatively good indicators to evaluate the level of seedling vigour in rice.
Figures

References
-
- Bertin P, Kinet JM, Bouharmont J. 1996. Evaluation of chilling sensitivity in different rice varieties. Relationship between screening procedures applied during germination and vegetative growth. Euphytica 89: 201–210.
-
- Chapman AL, Peterson ML. 1962. The seedling establishment of rice under water in relating to temperature and dissolved oxygen. Crop Science 2: 391–395.
-
- Cui KH, Peng SB, Xing YZ, Xu CG, Yu SB, Zhang Q. 2002. Molecular dissection of seedling-vigour and associated physiological traits in rice. Theoretical and Applied Genetics 105: 745–753. - PubMed
-
- Dingkuhn M, De Datta SK, Pamplona R, Javellana C, Schnier HF. 1992. Effect of late-season N-fertilization on photosynthesis and the yield of transplanted and direct-seeded tropical flooded rice. 2. A canopy stratification study. Field Crops Research 28: 235–249.