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. 2004 Dec;42(12):5899-903.
doi: 10.1128/JCM.42.12.5899-5903.2004.

Study of molecular epidemiology of candidiasis in portugal by PCR fingerprinting of Candida clinical isolates

Alexandra Correia  1 , Paula Sampaio, Judite Almeida, Célia Pais
Affiliations

Study of molecular epidemiology of candidiasis in portugal by PCR fingerprinting of Candida clinical isolates

Alexandra Correia et al. J Clin Microbiol. 2004 Dec.

Abstract

PCR fingerprinting was used to type 177 yeast isolates obtained from two medical institutions. Candida albicans was the predominant species found, followed by C. tropicalis, C. glabrata, C. parapsilosis, C. guilliermondii, and C. krusei, which accounted for over 20% of the strains isolated. This survey represents the first study of molecular epidemiology of candidiasis in Portugal.

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Figures

FIG. 1.
FIG. 1.
PCR profiles obtained with primer T3B for C. glabrata CBS 138 (1), C. parapsilosis CBS 604 (2), C. tropicalis PYCC 3097 (3), C. krusei PYCC 3440 (4), C. guilliermondii PYCC 2730 (5), C. lusitaniae PYCC 2705 (6), and C. albicans PYCC 3436 (7). M, molecular size marker in base pairs.
FIG. 2.
FIG. 2.
PCR profiles obtained with primer T3B for C. parapsilosis (lanes 1 and 2); Candida sp. strain 36 M (3), strain 65 M (4), strain 66 M (5), and strain 153 M (6); and C. glabrata (lanes 7 to 10). M, molecular size marker in base pairs.
FIG. 3.
FIG. 3.
PCR profiles obtained with primer T3B for C. albicans (lanes 1 and 2) and C. dubliniensis (lanes 3 and 4). M, molecular size marker in base pairs.
FIG. 4.
FIG. 4.
Dendrogram showing the degree of similarity of T3B fingerprinting profiles among the clinical Candida isolates by using the Dice coefficient and UPGMA cluster method. The unidentified strains are indicated as follows: *, 36 M, 65 M, and 66 M; **, 153 M. An arbitrary line has been drawn at 0.58 delimitating the major groups. r, cophenetic correlation coefficient.
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