[Molecular basis of Mycoplasma agalactiae pathogenicity]

Abstract

Compared to other bacterial pathogens, the current knowledge of the molecular basis of pathogenicity of mycoplasmas is limited, and their strategies of infection at the molecular and cellular level remain to be elucidated. Several studies in the past years have shown that pathogenic mycoplasmas are equipped with sophisticated genetic systems, which allow these agents to spontaneously change their surface antigenic make-up. It is implicated that these variable surface components provide the wall-less mycoplasmas with a means to avoid the host immune response and promote host colonization. In Mycoplasma (M.) agalactiae, the agent of "contagious agalactia" in sheep and goats, a pathogenicity island-like locus has recently been identified that contains six distinct but related genes which encode the major immunodominant membrane proteins, the so-called Vpmas. It was shown that these surface-associated proteins vary in expression at an unusual high frequency due to site-specific DNA rearrangements. The previous lack of tools to genetically manipulate M. agalactiae has hampered more refined studies to assess the exact function of Vpmas in M. agalactiae infection and disease. The recent successful introduction of foreign DNA into the M. agalactiae genome therefore represents an important breakthrough which sets up the basis for a variety of follow-up studies assessing the role of Vpmas in molecular pathogenesis.