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. 2004 Sep;21(9):329-34.
doi: 10.1023/b:jarg.0000045472.76766.d4.

Effects of activation timing on the fertilization rate and early embryo development in porcine ROSI procedure

Jong Yeob Choi  1 , Eun Young Lee, Hee Tae Cheong, Byung Koo Yoon, Duk Soo Bae, Doo Seok Choi
Affiliations

Effects of activation timing on the fertilization rate and early embryo development in porcine ROSI procedure

Jong Yeob Choi et al. J Assist Reprod Genet. 2004 Sep.

Abstract

Purpose: This study was undertaken to evaluate the optimal exposure time for nuclear envelope breakdown (NEBD) after injection of a round spermatid and to investigate the effect of oocyte activation timing on the fertilization rate and early embryo development in porcine round spermatid injection procedure.

Methods: Injected oocytes were fixed at 0.5, 1, 2, 3, and 4 h before activation, and NEBD state was examined. The three groups of oocytes were activated before and after the injection of spermatid using a single direct current pulse (100 V/mm, 50 micros): group 1) at 2 h before the injection (pre), group 2) within 0.5 h after the injection (immediate), and group 3) at 2 h after the injection (post). Activated oocytes were cultured and pronucleus formation and blastocyst development was evaluated at 15-18 h and 7-8 days after the injection, respectively.

Results: The proportion of oocytes with NEBD significantly increased in the groups with over 2 h of exposure time (p < 0.05) and oocyte with premature chromosome condensation began to appear 3 h after the injection. Normal fertilization and development rate to the blastocyst stage were significantly higher in the post group than in those of the pre or immediate group (p < 0.05).

Conclusion: The optimal exposure time for NEBD is 2 h after the injection, and activation in 2 h after round spermatid injection improved the normal fertilization and early embryo development rate.

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