Protection of dairy cows immunized with tick tissues against natural Boophilus microplus infestations in Thailand

Abstract

Boophilus microplus has a major impact on cattle production, and an antitick vaccine would be a valuable tool for control of this important ectoparasite in Thailand. Previous work has shown that immunization of hosts with different tick tissues has different implications regarding tick feeding and fecundity under experimental conditions. The purpose of this study was to assess the effects of immunization of dairy cattle with B. microplus salivary gland or midgut extracts on natural infestations by this tick species. The different antigen extracts (1 mg total protein) or equivalent amounts of adjuvant alone were injected intradermally every two weeks for a total of three times before allowing cattle to graze in a tick-contaminated pasture. Animals were checked daily, and engorged female ticks collected, counted, weighed, and maintained in tick incubators to observe tick performance parameters, including engorged weight, egg mass weight, nonviable eggs, mortality, oviposition period, egg incubation period, and F1 larval weight. After six months, each group was reimmunized with the same antigen and/or adjuvant, and ticks were again collected and evaluated. Immunization of cattle with salivary gland preparations resulted in reductions in mean tick counts and in engorged female weights. Immunization with midgut antigens reduced tick oviposition and reduced egg mass weights. In addition, more ticks recovered from midgut-immunized cows produce nonviable eggs. This investigation indicates that a vaccine based on these antigen preparations could induce a lasting, protective immune response against B. microplus that would be expected to provide a safe nontoxic means of tick control.