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. 2005 Jan 1;33(Database issue):D338-43.
doi: 10.1093/nar/gki047.

Pseudomonas aeruginosa Genome Database and PseudoCAP: facilitating community-based, continually updated, genome annotation

Geoffrey L Winsor  1 , Raymond Lo, Shannan J Ho Sui, Korine S E Ung, Shaoshan Huang, Dean Cheng, Wai-Kay Ho Ching, Robert E W Hancock, Fiona S L Brinkman
Affiliations

Pseudomonas aeruginosa Genome Database and PseudoCAP: facilitating community-based, continually updated, genome annotation

Geoffrey L Winsor et al. Nucleic Acids Res. .

Abstract

Using the Pseudomonas aeruginosa Genome Project as a test case, we have developed a database and submission system to facilitate a community-based approach to continually updated genome annotation (http://www.pseudomonas.com). Researchers submit proposed annotation updates through one of three web-based form options which are then subjected to review, and if accepted, entered into both the database and log file of updates with author acknowledgement. In addition, a coordinator continually reviews literature for suitable updates, as we have found such reviews to be the most efficient. Both the annotations database and updates-log database have Boolean search capability with the ability to sort results and download all data or search results as tab-delimited files. To complement this peer-reviewed genome annotation, we also provide a linked GBrowse view which displays alternate annotations. Additional tools and analyses are also integrated, including PseudoCyc, and knockout mutant information. We propose that this database system, with its focus on facilitating flexible queries of the data and providing access to both peer-reviewed annotations as well as alternate annotation information, may be a suitable model for other genome projects wishing to use a continually updated, community-based annotation approach. The source code is freely available under GNU General Public Licence.

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Figures

Figure 1
Figure 1
Schematic overview of the Pseudomonas Genome Database and PseudoCAP annotation update process. Boxes with thick lines/borders denote database and application components. Ovals reflect processes in the annotation update approach. Elements with thin lines/borders reflect human intervention.
See this image and copyright information in PMC

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