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. 2005 Jan 7;11(1):51-5.
doi: 10.3748/wjg.v11.i1.51.

Point mutation of 5' noncoding region of BCL-6 gene in primary gastric lymphomas

Da-Liu Min  1 , Xiao-Yan Zhou, Wen-Tao Yang, Hong-Fen Lu, Tai-Ming Zhang, Ai-Hua Zhen, Pei-Zheng Cao, Da-Ren Shi
Affiliations

Point mutation of 5' noncoding region of BCL-6 gene in primary gastric lymphomas

Da-Liu Min et al. World J Gastroenterol. .

Abstract

Aim: To investigate the mutations of the 5' noncoding region of BCL-6 gene in Chinese patients with primary gastric lymphomas.

Methods: PCR and direct DNA sequencing were used to identify BCL-6 gene mutations in the 5' noncoding region in 29 cases of gastric diffuse large B-cell lymphoma (DLBCL) and 18 cases of gastric mucosa-associated lymphoid tissue (MALT) lymphoma as well as 10 cases of reactive hyperplasia of lymph node (LRH).

Results: Six of 29 gastric DLBCLs (20.7%), 4 of 18 gastric MALT lymphomas (22.2%) and 1 of 10 LRHs(10%) were found to have mutations. All mutations were single-base substitutions and the frequency of single-base changes was 0.20 x 10(-2) -1.02 x 10(-2) per bp.

Conclusion: Point mutations in the 5' noncoding region of BCL-6 gene are found in Chinese patients with primary gastric DLBCLs and MALT lymphomas, suggesting that they may, in some extent, participate in the pathogenesis of primary gastric DLBCLs and MALT lymphomas.

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Figures

Figure 1
Figure 1
Amplified E1.11 (lanes 1-4) (215 bp) and E1.12 (lanes 5-8) (295 bp) fragments of BCL-6 gene shown by 2% agarose gel electrophoresis.
Figure 2
Figure 2
Representative results of direct DNA sequencing of PCR product E1.11 in primary gastric DLBCL. A: Wild-type BCL-6 gene; B: Mutated-type BCL-6 gene, arrow points to point mutation (G→C).
See this image and copyright information in PMC

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