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. 2005 Jan 7;11(1):69-72.
doi: 10.3748/wjg.v11.i1.69.

Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma

Hong-Bin Cui  1 , Xiao-Lin Na, Dan-Feng Song, Ying Liu
Affiliations

Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma

Hong-Bin Cui et al. World J Gastroenterol. .

Abstract

Aim: To study the blocking effects of genistein on cell proliferation cycle in human gastric carcinoma cells (SGC-7901) and the possible mechanism.

Methods: MTT assay was applied in the detection of the inhibitory effects of genistein on cell proliferation. Flow cytometry was used to analyze the cell cycle distribution. Immunocytochemical technique and Western blotting were performed to detect the protein expression of cyclin D1, cyclin B1 and p21(waf1/cip1).

Results: Genistein significantly inhibited the growth and proliferation of human gastric carcinoma cells (SGC-7901). Seven days after treatment with different concentrations of genistein (2.5, 5.0, 10.0, 20.0 microg/mL), the growth inhibitory rates were 11.2%, 28.8%, 55.3%, 84.7% respectively and cell cycles were arrested at the G(2)/ M phase. Genistein decreased cyclin D1 protein expression and enhanced cyclin B1 and p21(waf/cip1) protein expression in a concentration-dependent manner.

Conclusion: The growth and proliferation of SGC-7901 cells can be inhibited by genistein via blocking the cell cycle, with reduced expression of cyclin D1 and enhanced expression of cyclin B1 and p21(waf/cip1) protein in the concentration range of 0-20 microg/mL.

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Figures

Figure 1
Figure 1
Inhibitory effect of genistein on growth of SGC-7901 cells. The cells were treated with various concentrations of genistein for 1-7 d, the antiproloferative effect was measured by MTT assay. Results were expressed as mean±SD from 4 wells.
Figure 2
Figure 2
Expression of p21waf1/cip1 protein after treated with different genistein concentrations for 48 h.
Figure 3
Figure 3
Calculation of areas of p21waf1/cip1 protein by ChemiImager 4000.
See this image and copyright information in PMC

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