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. 2004 May;2(2):109-18.
doi: 10.1016/s1672-0229(04)02016-9.

Identification and characterization of 177 unreported genes associated with liver regeneration

Cunshuan Xu  1 , Cuifang Chang, Jinyun Yuan, Hongpeng Han, Kejin Yang, Lifeng Zhao, Wenqiang Li, Yuchang Li, Huiyong Zhang, Salman Rahman, Jingbo Zhang
Affiliations

Identification and characterization of 177 unreported genes associated with liver regeneration

Cunshuan Xu et al. Genomics Proteomics Bioinformatics. 2004 May.

Abstract

The mammalian liver has a very strong regeneration capacity after partial hepatectomy (PH). To further learn the genes participating in the liver regeneration (LR), 551 cDNAs selected from subtracted cDNA libraries of the regenerating rat liver were screened by microarray, and their expression profiles were studied by cluster and generalization analyses. Among them, 177 genes were identified unreported and up- or down-regulated more than twofold at one or more time points after PH, of which 62 genes were down-regulated to less than 0.5; 99 genes were up-regulated to 2-10 folds, and 16 genes were either up- or down-regulated at different time points during LR. By using BLAST and GENSCAN, these genes were located on responsible chromosomes with 131 genes on the long arms of the chromosomes. The cluster and generalization analyses showed that the gene expression profiles are similar in 2 and 4, 12 and 16, 96 and 144 h respectively after PH, suggesting that the actions of the genes expressed in the same profiles are similar, and those expressed in different profiles have less similarity. However, the types, characteristics and functions of the 177 genes remain to be further studied.

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Figures

Fig. 1
Fig. 1
Genes expressed at different time points of liver regeneration.
Fig. 2
Fig. 2
Hierarchical cluster analysis of 177 genes. A. Cluster of distribution trend. 177 genes differing with more than twofold intensity at least one time point of liver regeneration were identified; B. Cluster of hierarchical relativity at eleven time points.
Fig. 3
Fig. 3
The 177 genes expressed at different time points after PH are categorized into 8 patterns based on their similarity, that is, 2 and 4, 8, 12 and 16, 24, 36, 48, 72, 96 and 144 h.
See this image and copyright information in PMC

References

    1. Michalopoulos G.K. Liver regeneration. Science. 1997;276:60–66. - PubMed
    1. Fausto N. Liver regeneration. J. Hepatol. 2000;32:19–31. - PubMed
    1. Zimmermann A. Liver regeneration: the emergence of new pathways. Med. Sci. Moint. 2002;8:53–63. - PubMed
    1. Satyanarayana A. Cell Cycle. 2004. Gene expression profile at the G(1)/S transition of liver regeneration after partial hepatectomy in mice. In press. - PubMed
    1. Xu C.S. Effects of the short interval successive partial hepatectomy on rat survival and liver tissue structure. Acta Zool. Sin. 2001;47:659–665.

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