Interactions and trafficking of Andes and Sin Nombre Hantavirus glycoproteins G1 and G2

Abstract

This study was designed to investigate the trafficking of Andes virus (ANDV) and Sin Nombre virus (SNV) glycoproteins and to determine if ANDV or SNV glycoproteins G1 and G2 could be substituted for each other while still retaining normal trafficking. Trafficking of Hantaan virus (HNTV) and SNV glycoproteins has been studied and conflicting results were published regarding the Golgi targeting of G1 and G2 when expressed individually. The results reported in this manuscript suggest that both SNV and ANDV G1 and G2 expressed together, either from a single glycoprotein precursor (GPC) or from separate cDNAs, co-localize to the Golgi complex (GC). When expressed individually, neither G1 nor G2 was able to translocate from the endoplasmic reticulum (ER) to the GC. Interestingly, when ANDV G1 and SNV G2 or ANDV G2 and SNV G1 are co-expressed, they interact and are colocalized in the GC.