Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Multicenter Study
. 2005 Jan;43(1):127-31.
doi: 10.1128/JCM.43.1.127-131.2005.

Ability of new APTIMA CT and APTIMA GC assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in male urine and urethral swabs

M A Chernesky  1 , D H Martin, E W Hook, D Willis, J Jordan, S Wang, J R Lane, D Fuller, J Schachter
Affiliations
Multicenter Study

Ability of new APTIMA CT and APTIMA GC assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in male urine and urethral swabs

M A Chernesky et al. J Clin Microbiol. 2005 Jan.

Abstract

A clinical evaluation was conducted in six North American centers to determine the ability of APTIMA CT (ACT) and APTIMA GC (AGC) nucleic acid amplification assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections in 1,322 men by testing their urethral swabs and first-catch urine (FCU). The results obtained with ACT and AGC assays were compared to an infected patient status determined by testing the specimens with the APTIMA Combo 2 and the BD ProbeTec energy transfer multiplex assays. Symptoms did not influence the values. Positive and negative agreements of the ACT and AGC assays for individual specimens were high, with each comparator assay ranging between 94.3 and 100% for positives and 93.9 and 99.4% for negatives. The ACT and AGC assays performed on noninvasive specimens such as FCU effectively identified C. trachomatis or N. gonorrhoeae infections in symptomatic and asymptomatic men and should be suitable for screening male populations.

PubMed Disclaimer

References

    1. Bauwens, J. E., A. M. Clark, M. J. Loeffelholz, S. A. Herman, and W. E. Stamm. 1993. Diagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine. J. Clin. Microbiol. 31:3013-3016. - PMC - PubMed
    1. Cates, W., and J. N. Wasserheit. 1991. Genital chlamydia infections: epidemiology and reproductive sequelae. Am. J. Obstet. Gynecol. 164:1771-1781. - PubMed
    1. Chernesky, M., H. Lee, J. Schachter, J. D. Burczak, W. E. Stamm, W. M. McCormack, and T. C. Quinn. 1994. Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay. J. Infect. Dis. 170:1308-1311. - PubMed
    1. Chernesky, M. A., S. Chong, D. Jang, K. Luinstra, J. Sellors, and J. B. Mahony. 1997. Ability of commercial ligase chain reaction and PCR assays to diagnose Chlamydia trachomatis infections in men by testing first-void urine. J. Clin. Microbiol. 35:982-984. - PMC - PubMed
    1. Chong, S., D. Jang, X. Song, J. Mahony, A. Petrich, P. Barriga, and M. Chernesky. 2003. Specimen processing and concentration of Chlamydia trachomatis added can influence false-negative rates in the LCx assay but not in the APTIMA Combo 2 assay when testing for inhibitors. J. Clin. Microbiol. 41:778-782. - PMC - PubMed