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. 2005 Jan;43(1):448-51.
doi: 10.1128/JCM.43.1.448-451.2005.

PCR amplification with primers based on IS2404 and GC-rich repeated sequence reveals polymorphism in Mycobacterium ulcerans

Anthony Ablordey  1 , Roman Kotlowski, Jean Swings, Françoise Portaels
Affiliations

PCR amplification with primers based on IS2404 and GC-rich repeated sequence reveals polymorphism in Mycobacterium ulcerans

Anthony Ablordey et al. J Clin Microbiol. 2005 Jan.

Abstract

We describe a simple genotyping method for Mycobacterium ulcerans based on PCR amplification of genomic regions between IS2404 and a frequently repeated GC-rich sequence. Application of this method to a global collection produced 10 M. ulcerans genotypes corresponding to their geographic origin.

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Figures

FIG. 1.
FIG. 1.
IS2404-Mtb2 PCR analysis of M. ulcerans isolates from different geographic regions and isolate ITM 00-1026, the missing link. (A) Lanes: L, DNA ladder; 1, ITM 5142; 2, ITM 1326; 3, ITM 1329; 4, ITM 9540; 5, ITM 00-1026; 6, ITM 9537; 7, ITM 94-1331; 8, ITM 98-912; 9, ITM 8756; 10, ITM 94-1328; 11, ITM 5143; 12, ITM 5114. (B) Lanes: L, DNA ladder; 1, ITM 842; 2, ITM 7922; 3, ITM 5150; 4, ITM 5155; 5, ITM 96-658; 6, ITM 96-657; 7, ITM 97-610; 8, ITM 02-279; 9, ITM 00-0040; 10, ITM 00-1213; 11, ITM 00-1441; 12, ITM 94-662. The 15- and 1,500-bp bands in all lanes represent lower and upper internal markers.
FIG. 2.
FIG. 2.
Dendrogram showing relationships among the 33 isolates. Cluster analysis was performed by the unweighted pair group method, using the arithmetic average algorithm with the Bionumerics version 3.0 computer software (Sint-Martens-Latem, Belgium). Percent similarity was calculated with the band-based Dice correlation coefficient. The numbers in parentheses represent the number of isolates tested.
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References

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