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Comparative Study
. 2005 Jan;43(1):506-8.
doi: 10.1128/JCM.43.1.506-508.2005.

Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings

Richard A Respess  1 , Ada Cachafeiro, David Withum, Susan A Fiscus, Daniel Newman, Bernard Branson, Oliviero E Varnier, Kim Lewis, Timothy J Dondero
Affiliations
Comparative Study

Evaluation of an ultrasensitive p24 antigen assay as a potential alternative to human immunodeficiency virus type 1 RNA viral load assay in resource-limited settings

Richard A Respess et al. J Clin Microbiol. 2005 Jan.

Abstract

An inexpensive enzyme-linked immunosorbent assay method for human immunodeficiency virus type 1 quantitation, ultrasensitive p24 antigen assay (Up24), was compared with RNA viral load assay (VL). Up24 had 100% sensitivity of detection at a viral load of >/=30,000, with sensitivity of 46.4% at a viral load of <30,000 (232 specimens from 65 seropositive subjects). The assay was highly reproducible, with excellent correlation between duplicates and among three laboratories.

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Figures

FIG. 1.
FIG. 1.
Correlation of Up24 and VL for 65 seropositive adult U.S. patients, either untreated (n = 83; ) or ARV treated (n = 149; ▴).
See this image and copyright information in PMC

References

    1. Boni, J., O. Opravil, Z. Tomasik, M. Rothen, L. Bisset, P. J. Grob, R. Luthy, and J. Schupbach. 1997. Simple monitoring of antiretroviral therapy with a signal-amplification-boosted HIV-1 p24 antigen assay with heat-denatured plasma. AIDS 11:F47-F52. - PubMed
    1. Braun, J., J-C Plantier, M-F Hellot, E. Tuaillon, M. Gueudin, F. Damond, A. Malmsten, G. E. Corrigan, and F. Simon. 2003. A new quantitative HIV load assay based on plasma virion reverse transcriptase activity for the different types, groups and subtypes. AIDS 17:331-336. - PubMed
    1. Burgisser, P. P. Vernazza, M. Flepp, J. Boni, Z. Tomasik, U. Hummel, G. Pantaleo, and J. Schupbach. 2000. Performance of five different assays for the quantification of viral load in subjects infected with various subtypes of HIV-1. J. Acquired Immune Defic. Syndr. 23:138-144. - PubMed
    1. Giacomini, M., J. L. McDermott, A. A. Giri, I. Martini, F. B. Lillo, and O. E. Varnier. 1998. A novel and innovative quantitative kinetic software for virologic colorimetric assays. J. Virol. Methods 73:201-209. - PubMed
    1. Goldschmidt, P. L., A. Devillechabrolle, Z. Ai-Arkoub, and J. T. Aubin. 1998. Comparison of an amplified enzyme-linked immunosorbent assay with procedures based on molecular biology for assessing human immunodeficiency virus type 1 viral load. Clin. Diagn. Lab. Immunol. 5:513-518. - PMC - PubMed