Adherence of Nocardia asteroides within the murine brain

Abstract

Nonlethal infection of BALB/c mice with Nocardia asteroides GUH-2 (GUH-2) produces a variety of neurological signs, including an L-dopa-responsive movement disorder in 10 to 15% of the infected population. To study nocardial interactions with the brain, we characterized the attachment of GUH-2 within specific regions through the use of microdissection. Following an intravenous injection of a single-cell suspension of log-phase GUH-2, viable cells were recovered from all regions of the brain, and the distribution of the nocardiae was independent of the size of the inoculum. In addition, two mutants of GUH-2 were found to possess significantly altered binding characteristics with regard to both the percentage of the inoculum bound per brain and the relative distribution of adherence to regions of the brain, when compared with the parental strain. These results indicated that GUH-2 bound throughout the murine brain and suggested that GUH-2 utilized specific receptors to facilitate this attachment.