Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Jan 28;11(4):577-9.
doi: 10.3748/wjg.v11.i4.577.

Inhibitory effects of polysaccharides isolated from Phellinus gilvus on benzo(a)pyrene-induced forestomach carcinogenesis in mice

Jae-Sung Bae  1 , Kwang-Ho Jang, Hyunee Yim, Seung-Chun Park, Hee-Kyung Jin
Affiliations

Inhibitory effects of polysaccharides isolated from Phellinus gilvus on benzo(a)pyrene-induced forestomach carcinogenesis in mice

Jae-Sung Bae et al. World J Gastroenterol. .

Abstract

Aim: Although polysaccharides from Phellinus mushrooms are a well-known material with anti-tumor properties, there is no information about the effect of polysaccharides from Phellinus gilvus (PG) on tumor. The modulating effect of polysaccharides isolated from PG on the benzo(a)pyrene (BaP)-induced forestomach carcinogenesis in ICR female mice was investigated in this study.

Methods: A forestomach carcinogenesis model was established in 40 ICR female mice receiving oral administration of BaP for 4 wk. The mice were randomly assigned to 4 groups (10 each). The mice in each group were treated with sterile water or PG for 4 and 8 wk (SW4, PGW4, SW8, and PGW8 groups). Eight or 12 wk after the first dose of BaP, forestomachs were removed for histopathological and RT-PCR analysis.

Results: In histopathological changes and RT-PCR analysis, sterile water-treated mice showed significant hyperplasia of the gastric mucosa with a significantly increased expression of mutant p53 mRNA compared to mice treated with PG for 8 wk.

Conclusion: Polysaccharides isolated from PG may inhibit BaP-induced forestomach carcinogenesis in mice bydown-regulating mutant p53 expression.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Histopathologic changes of forestomachs according to each treatment in BaP-induced forestomach carcinogenesis (n = 5/each group). Note the significantly reduced hyperplasia of the gastric mucosa in PGW8 group, compared with SW groups. A: CO group (normal mucosa of forestomach); B: SW4 group; C: SW8 group; D: PGW4 group; E: PGW8 group (×200).
Figure 2
Figure 2
p53 mRNA expression in BaP-induced forestomach carcinogenesis by RT-PCR analysis (n = 5/each group). The p53 gene expression was greatly down-regulated in PGW8 group, compared to the SW groups. The b-actin transcript levels among all groups were the same. A: CO group, B: SW4 group, C: SW8 group, D: PGW4 group, E: PGW8 group.
See this image and copyright information in PMC

References

    1. Parkin DM, Pisani P, Ferlay J. Estimates of the worldwide incidence of 25 major cancers in 1990. Int J Cancer. 1999;80:827–841. - PubMed
    1. Wu K, Shan YJ, Zhao Y, Yu JW, Liu BH. Inhibitory effects of RRR-alpha-tocopheryl succinate on benzo(a)pyrene (B(a)P)-induced forestomach carcinogenesis in female mice. World J Gastroenterol. 2001;7:60–65. - PMC - PubMed
    1. Jagetia GC, Baliga MS, Venkatesh P. Effect of Sapthaparna (Alstonia scholaris Linn) in modulating the benzo(a)pyrene-induced forestomach carcinogenesis in mice. Toxicol Lett. 2003;144:183–193. - PubMed
    1. Han SB, Lee CW, Jeon YJ, Hong ND, Yoo ID, Yang KH, Kim HM. The inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacology. 1999;41:157–164. - PubMed
    1. Bae JS, Hwang MH, Jang KH, Rhee MH, Lee KW, Jo WS, Choi SG, Yun HI, Yim JH, Kim JC, et al. Comparative antitumor activity of water extracts from fruiting body of Phellinus linteus, Phellinus baumii and Phellinus gilvus. J Toxicol Pub Health. 2004;20:37–42.

Publication types

MeSH terms