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. 2005 Jan 11:2:1.
doi: 10.1186/1742-4682-2-1.

Human embryonal epithelial cells of the developing small intestinal crypts can express the Hodgkin-cell associated antigen Ki-1 (CD30) in spontaneous abortions during the first trimester of gestation

Demetrio Tamiolakis  1 , John Venizelos, Maria Lambropoulou, Silva Nikolaidou, Sophia Bolioti, Maria Tsiapali, Dionysios Verettas, Panagiotis Tsikouras, Athanasios Chatzimichail, Nikolas Papadopoulos
Affiliations

Human embryonal epithelial cells of the developing small intestinal crypts can express the Hodgkin-cell associated antigen Ki-1 (CD30) in spontaneous abortions during the first trimester of gestation

Demetrio Tamiolakis et al. Theor Biol Med Model. .

Abstract

Background: Ki-1 (CD30) antigen expression is not found on peripheral blood cells but its expression can be induced in vitro on T and B lymphocytes by viruses and lectins. Expression of CD30 in normal tissues is very limited, being restricted mainly to a subpopulation of large lymphoid cells; in particular, cells of the recently described anaplastic large cell lymphoma (ALCL), the Reed-Sternberg (RS) cells of Hodgkin's lymphoma and scattered large parafollicular cells in normal lymphoid tissues. More recent reports have described CD30 expression in non-hematopoietic and malignant cells such as cultured human macrophages, human decidual cells, histiocytic neoplastic cells, mesothelioma cells, embryonal carcinoma and seminoma cells.

Results: We investigated the immunohistochemical expression of CD30 antigen in 15 paraffin-embedded tissue samples representing small intestines from fetuses after spontaneous abortion in the 8th, 10th and 12th weeks using the monoclonal antibody Ki-1. Hormones had been administered to all our pregnant women to support gestation. In addition, a panel of monoclonal antibodies was used to identify leukocytes (CD45/LCA), B-lymphocytes (CD20/L-26) and T-lymphocytes (CD3). Our findings were correlated with those obtained simultaneously from intestinal tissue samples obtained from 15 fetuses after therapeutic or voluntary abortions.

Conclusions: The results showed that: (1) epithelial cells in the developing intestinal crypts express the CD30 (Ki-1) antigen; (2) CD30 expression in these epithelial cells is higher in cases of hormonal administration than in normal gestation. In the former cases (hormonal support of gestation) a mild mononuclear intraepithelial infiltrate composed of CD3 (T-marker)-positive cells accompanies the CD30-positive cells.

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Figures

Figure 1
Figure 1
8th week of gestation (involuntary abortions). Ki-1 (CD30) antigen is expressed by a small number of epithelial cryptae cells. Immunohistochemical stain X 400.
Figure 2
Figure 2
8th week of gestation (voluntary abortions). Weak to moderate expression of Ki-1 (CD30) antigen in the developing crypts. Immunohistochemical stain X 400.
Figure 3
Figure 3
10th week of gestation (involuntary abortions). Strong expression of Ki-1 (CD30) antigen in the developing crypts. Immunohistochemical stain X 400
See this image and copyright information in PMC

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  • Ber-H2 (CD30) immunohistochemical staining of human fetal tissues.
    Tamiolakis D, Papadopoulos N, Lambropoulou M, Venizelos J, Verettas D, Tsikouras P, Koutsougeras G, Papadopoulos H, Karpouzis A, Kouskoukis C. Tamiolakis D, et al. Int J Biol Sci. 2005;1(4):135-40. doi: 10.7150/ijbs.1.135. Epub 2005 Oct 1. Int J Biol Sci. 2005. PMID: 16244703 Free PMC article.

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