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. 1992 Apr 10;256(5054):219-21.
doi: 10.1126/science.1566068.

Centriole duplication in lysates of Spisula solidissima oocytes

R E Palazzo  1 , E Vaisberg, R W Cole, C L Rieder
Affiliations

Centriole duplication in lysates of Spisula solidissima oocytes

R E Palazzo et al. Science. .

Erratum in

  • Science 1992 Jun 26;256(5065):1746

Abstract

A cell-free system has been developed that executes centriole duplication. Surf clam (Spisula solidissima) oocytes, arrested at late prophase of meiosis I, do not contain centrioles, centrosomes, or asters. Serial section high-voltage electron microscopy (HVEM) of asters and spindles isolated from potassium chloride-activated oocytes indicates that within 4 minutes oocytes assemble a single centriole that is duplicated by 15 minutes when assembly of the first meiotic spindle is complete. A mixture of lysates from unactivated oocytes and potassium chloride-activated oocytes induces centriole formation and duplication. Astral microtubule content in these lysate mixtures increases with time.

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