[Molecular techniques for detection, identification and analysis of human papillomaviruses (HPVs)]

Abstract

The review presents the main molecular virology techniques for detection, identification and analysis of human papillomaviruses (HPVs). Theoretical and practical bases of hybridization techniques (Southern blot, Dot blot, In situ hybridization, Filter in situ hybridization FISH, Hybrid Capture Assay, HCA), amplification techniques (polymerase chain reaction, PCR and variants--multiplex PCR, reverse transcription PCR, real-time PCR) and sequencing techniques(sequencing analysis, single-strand conformation polymorphism--SSCP) are discussed.