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. 2005 Feb;49(2):804-7.
doi: 10.1128/AAC.49.2.804-807.2005.

Simple fibroblast-based assay to test the pyrazinamide susceptibility of Mycobacterium tuberculosis

Takemasa Takii  1 , Sonomi Hamasaki, Kazue Hirano, Chiyoji Abe, Kikuo Onozaki
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Simple fibroblast-based assay to test the pyrazinamide susceptibility of Mycobacterium tuberculosis

Takemasa Takii et al. Antimicrob Agents Chemother. 2005 Feb.

Abstract

A simple fibroblast-based assay (SFA) was found to be efficient in evaluating the susceptibilities of clinical isolates of Mycobacterium tuberculosis to pyrazinamide (PZA). Forty-five clinical isolates were examined. The MICs of PZA for susceptible strains in an SFA were between 3.13 and 12.5 microg/ml, and the MICs of PZA for resistant strains were more than 100 microg/ml.

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