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. 2005 Feb;49(2):824-6.
doi: 10.1128/AAC.49.2.824-826.2005.

Elucidation of essential and nonessential genes in the Haemophilus influenzae Rd cell wall biosynthetic pathway by targeted gene disruption

Catherine M Trepod  1 , John E Mott
Affiliations

Elucidation of essential and nonessential genes in the Haemophilus influenzae Rd cell wall biosynthetic pathway by targeted gene disruption

Catherine M Trepod et al. Antimicrob Agents Chemother. 2005 Feb.

Abstract

Targeted gene disruption by in vitro transposon mutagenesis has been used to identify the genes required for biosynthesis of the Haemophilus influenzae Rd cell wall under standard cultivation conditions. Of the 28 genes known to be associated with the cell wall biosynthetic pathway, 14 were determined to be essential.

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Figures

FIG. 1.
FIG. 1.
The H. influenzae cell wall biosynthetic pathway. Essential enzymes are underlined and in bold. Nonessential enzymes are indicated by asterisks.
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