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. 2005 Feb 14;11(6):875-9.
doi: 10.3748/wjg.v11.i6.875.

Activation of STAT3 signaling in human stomach adenocarcinoma drug-resistant cell line and its relationship with expression of vascular endothelial growth factor

Li-Fen Yu  1 , Ying Cheng, Min-Min Qiao, Yong-Ping Zhang, Yun-Lin Wu
Affiliations

Activation of STAT3 signaling in human stomach adenocarcinoma drug-resistant cell line and its relationship with expression of vascular endothelial growth factor

Li-Fen Yu et al. World J Gastroenterol. .

Abstract

Aim: To investigate the difference in activation of STAT3 signaling between two human stomach adenocarcinoma cell lines: 5-fluorouracil resistant cell line and its parental cell line, and to evaluate its relationship with the expression of vascular endothelial growth factor (VEGF).

Methods: Western blot and electrophoretic mobility shift assay (EMSA) were used to detect the expression of phospho-STAT3 protein and constitutive activation of STAT3 in two human stomach adenocarcinoma cell lines, 5-fluorouracil resistant cell line SGC7901/R and its parental cell line SGC7901, respectively. The mRNA expression of VEGF was analysed by semi-quantitative RT-PCR. The expressive intensity of VEGF protein was measured by immunocytochemistry.

Results: The expressions of phospho-STAT3 protein and constitutive activation of STAT3 between two human stomach adenocarcinoma cell lines were different. Compared with the parental cell line SGC7901, the STAT3-DNA binding activity and the expressive intensity of phospho-STAT3 protein were lower in the drug-resistant cell line SGC7901/R. The expression levels of VEGF mRNA and its encoded protein were also decreased in drug-resistant cell line.

Conclusion: Over-expression of VEGF may be correlated with elevated STAT3 activation in parental cell line. Lower VEGF expression may be correlated with decreased STAT3 activation in resistant cell line, which may have resulted from negative feedback regulation of STAT signaling.

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Figures

Figure 1
Figure 1
Western blot analysis of phospho-STAT3 in different cell lines. Lane 1: SGC7901 cell line; lane 2: SGC7901/R cell line.
Figure 2
Figure 2
EMSA analysis of STAT3 DNA-binding activity in different cell lines. Lane 1: Competitive probe; lane 2: SGC7901/R cell line; lane 3: SGC7901 cell line.
Figure 3
Figure 3
Identification of VEGF mRNA by RT-PCR in different cell lines. M: Ladder marker; lane 1: SGC7901 cell line; lane 2: SGC7901/R cell line.
Figure 4
Figure 4
Immunocytochemical staining of VEGF in two human stomach adenocarcinoma cell lines (DAB×400). A: parental cell line SGC7901; B: drug-resistant cell line SGC7901/R.
See this image and copyright information in PMC

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References

    1. Bowman T, Garcia R, Turkson J, Jove R. STATs in oncogenesis. Oncogene. 2000;19:2474–2488. - PubMed
    1. Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE. Stat3 as an oncogene. Cell. 1999;98:295–303. - PubMed
    1. Grandis JR, Drenning SD, Zeng Q, Watkins SC, Melhem MF, Endo S, Johnson DE, Huang L, He Y, Kim JD. Constitutive activation of Stat3 signaling abrogates apoptosis in squamous cell carcinogenesis in vivo. Proc Natl Acad Sci USA. 2000;97:4227–4232. - PMC - PubMed
    1. Epling-Burnette PK, Liu JH, Catlett-Falcone R, Turkson J, Oshiro M, Kothapalli R, Li Y, Wang JM, Yang-Yen HF, Karras J, et al. Inhibition of STAT3 signaling leads to apoptosis of leukemic large granular lymphocytes and decreased Mcl-1 expression. J Clin Invest. 2001;107:351–362. - PMC - PubMed
    1. Bowman T, Broome MA, Sinibaldi D, Wharton W, Pledger WJ, Sedivy JM, Irby R, Yeatman T, Courtneidge SA, Jove R. Stat3-mediated Myc expression is required for Src transformation and PDGF-induced mitogenesis. Proc Natl Acad Sci USA. 2001;98:7319–7324. - PMC - PubMed

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