[Determination of scopoletin and umbelliferone contents in Saussurea medusa Maxim by high-performance liquid chromatography]
[Article in
Chinese]
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- PMID: 15684018
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[Determination of scopoletin and umbelliferone contents in Saussurea medusa Maxim by high-performance liquid chromatography]
[Article in
Chinese]
Di Yi Jun Yi Da Xue Xue Bao.
2005 Jan.
Abstract
The contents of scopoletin and umbelliferone in Saussurea medusa Maxim were determined by high-performance liquid chromatography (HPLC) using Merck Lichrospher100 RP-18e column (250 mm x 4.0 mm, 5 microm) with the mobile phase of methanol-tetrahydrofuran-water solution and the absorbance of the compounds observed at 346 nm. HPLC yielded an average recovery and RSD of 100.07% and 1.42% for scopoletin, and 99.41% and 2.06% for umbelliferone, respectively, suggesting that HPLC is a simple, rapid and accurate method for quality control of Saussurea medusa Maxim.
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