[Nude mouse model of human gastric carcinoma metastasis constructed by orthotopic transplantation using organism glue paste technique]

Abstract

Background & objective: Ideal animal model of tumor metastasis is an important precondition for the research on tumor prevention and treatment. Traditional gastric cancer models were constructed using full tumor tissue block by "subcutaneous transplantation" or "gastric bursa method" with limitations, such as operative complexity, and low survival rate. We tried to construct nude mouse model of human gastric carcinoma metastasis by orthotopic transplantation using organism glue paste technique to solve the problems.

Methods: Transplanted gastric tumor tissue block was constructed through repeatedly subcutaneously inoculating and passaging human gastric carcinoma SGC-7901 cells in nude mouse, and pasted orthotopically on the stomach wall of BALB/c nude mouse with OB biological glue. Tumor growth, successful rate of orthotopic transplantation, and incidence of metastasis in nude mice performed organism glue paste were compared with those in nude mice performed subcutaneous transplantation or gastric bursa method.

Results: In nude mice performed organism glue paste, the successful rate of orthotopic transplantation was 100%, the metastasis rate of local lymph node, lung, liver, and peritoneum were 100%, 62.5%, 87.5%, and 87.5%, respectively, while those in nude mice performed gastric bursa method were 100%, 100%, 50.0%, 50.0%, and 33.3%, respectively. The nude mice performed organism glue paste showed a increased metastatic rate of peritoneum (P < 0.05) with a higher survival rate. No local invasion and distance metastasis was observed in nude mice performed subcutaneous transplantation.

Conclusion: Organism glue paste technique can create gastric cancer metastasis model more easily than traditional methods, and represent clinical metastasis process.